- 中文名称
ACO1 |乌头酸酶
- 英文名字
- ACO1 | Aconitase
- 供应商
- Agrisera
- 产品货号
- AS09-521
- 产品报价
- ￥询价/100ul
- 产品说明书
- 点击查看
- 购买方式
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- 产品新闻
- 背景资料
- Aconitase is a single subunit enzyme of the tricarboxylic acid cycle (or Krebs cycle) in the mitochondria. A cytosolic isoform is also part of the glyoxylate cycle. Aconitase catalyzes the dehydration / hydration of citrate to iso-citrate, via cis-aconitate as an intermediate. The reaction is facilitated by an iron-sulphur cluster in the active site of the enzyme. The iron-sulphur cluster is somewhat unstable, especially under oxidative stress, and loss of the cofactor leads to degradation of the protein.Alternative names: ACO, citrate hydro-lyase 1,2,3
- 应用类型
- Western blot (WB)
- 免疫原
- Arabidopsis ACO1 (AT4G35830, Q42560), codon 120 - 898 (C-terminus), was cloned in fusion with a N-terminal 6xHis tag, and over-expressed in E. coli. All recombinant protein accumulated in inclusion bodies, which were purified by centrifugation and solubilised in 6 M guanidine-HCl. The protein was refolded by dilution in 100 mM Tris-HCl 8.5, 10% (v/v) glycerol, 2 mM dithiothreitol, and concentrated prior to immunisation.
- 来源宿主
- 兔
- 反应性
- 拟南芥 ACO1,ACO2 and ACO3 isoforms, Brassica oleracea, Solanum lycopersicum
- 保存建议
- Store lyophilized/reconstituted at -20
- 其他
- Agrisera公司提供1000种用于植物和藻类细胞生物学研究的一抗，2000多种二抗。Agrisera公司提供的植物领域的抗体涵盖拟南芥、莱茵衣藻、蓝藻、松柏类、硅藻、大豆、大麦、蒺藜苜蓿、烟草、稻、菜豆、小立碗藓、杨树、豌豆、番茄、马铃薯、菠菜、小麦、玉米、葡萄等多个物种，此外还有水生生物类（藻类、鱼）抗体，细菌、昆虫、真菌抗体。针对植物蛋白质的保守氨基酸序列，Agirsera还独家开发了数十种通用抗体（global antibodies），这类抗体几乎可以与所有的高等植物及藻类产生免疫反应，可为研究人员提供极为方便的选择。
- 注意
-
该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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