Ferritin is the major iron storage protein complex in eukaryotic cells, consisting of 24 ferritin subunit polypeptides. In Chlamydomonas reinhardtii 2 isoforms, ferritin1 (Fer1) and ferritin2 (Fer2) are present. Both are plastid localized but Fer1 is quantitatively recovered in soluble extracts of cells while Fer2 is found in the particulate fraction. Partial purification of the Fer1 complex indicates that the two ferritins are associated in distinct complexes and do not coassemble. The ratio of Fer1 to Fer2 in iron-replete cells is 70:1 and indicates a more dominant role of Fer1 in iron homeostasis.
应用类型
Western blot (WB)
免疫原
KLH-conjugated synthetic peptide derived from Fer2 protein sequence (A8WCT5) from Chlamydomonas reinhardtii
