Cofilin is ubiquitously expressed in eukaryotic cells where it binds to actin, thereby regulating the rapid cycling of actin assembly and disassembly essential for cellular viability . Cofilin is a low molecular weight protein that binds to filamentous (F) actin by bridging two longitudinally associated actin subunits changing the F-actin filament twist . This process is allowed by the dephosphorylation of cofilin ser-3 by factors such as opsonized zymosan . Lim kinase 1, a serine kinase, phosphorylates cofilin and renders it unable to bind and depolymerise F-actin .蛋白别名为:CFL1; CFL; Cofilin-1; 18 kDa phosphoprotein; p18; Cofilin; non-muscle isoform;基因ID为:3716;蛋白质ID:P23528
应用类型
WB,IHC-p,ELISA补充:最优的抗体稀释比例需要基于客户实验进行优化.建议的起始稀释比例如下: WB: 1:500-1:2000, IHC-p: 1:100-1:300, ELISA: 1:10000. Not yet tested in other applications.
免疫原
合成多肽:human Cofilin around the phosphorylation site of S3.