Intracellular pH plays an important modulating role in many cellular events, including cell growth, calcium regulation, enzymatic activity, receptor-mediated signal transduction, ion transport, endocytosis, chemotaxis, cell adhesion and other cellular processes。 pH-sensitive fluorescent dyes have been widely applied to monitor changes in intracellular pH in recent years。 Imaging techniques that use fluorescent pH indicators also allow researchers to investigate these processes with much greater spatial resolution and sampling density that can be achieved using other technologies such as microelectrode。 Among them, 2’,7’-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein (BCECF) is the most popular pH probe since it can be used to monitor cellular pH ratiometrically。 However, all the commercial BCECF AM is a complex mixture of at least three isomers with different ratios from batch to batch, complicating the BCECF applications。 BCFL is developed to overcome this isomer difficulty associated with BCECF AM。 As BCECF, BCFL exhibits a pH-dependent dual excitation, essentially identical to BCECF。 It has pKa of ~7。0, identical to BCECF too。 As with BCECF, the dual excitation spectrum of BCFL with an isosbestic point at 454 nm should make BCFL a good excitation-ratiometrie pH indicator。 BCFL ratiometric imaging makes intracellular pH determination essentially independent of several variable factors, including dye concentration, path length, cellular leakage and photobleaching rate。 BCFL, AM is a single isomer, making the pH measurement much more reproducible than the BCECF, AM, which is consisted of quite a few different isomers。
产品描述
RatioWorks BCFL, AM *pH荧光探针BCECF的卓越替代物*产品的光谱学相关参数为Ex(nm)=503/ Em(nm)528;其对应分子量大小为600。
产品形式
提供的RatioWorks BCFL, AM *pH荧光探针BCECF的卓越替代物*产品为固体形式,我们建议将其溶解到DMSO溶液中使用或保存。
保存建议
对于RatioWorks BCFL, AM *pH荧光探针BCECF的卓越替代物*产品,我们推荐使用常温进行运输。在您收到货物后,我们建议在低于-15℃温度下冷冻保存,要求储存环境干燥并避免光照。产品有效期为12个月。