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中文名称

Antithrombin (ATIII) BioAssay ELISA Kit (Antibodies only)

英文名字
Antithrombin (ATIII) BioAssay ELISA Kit (Antibodies only)
供应商
USBiological
产品货号
A2298-19
产品报价
¥1/1Kit
产品说明书
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产品新闻
背景资料
USBiological品牌是全球有名的抗原抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理,是行业中少有的致力于服务客户,帮助客户,且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应,专业的产品和解决方案支持,稳健快捷的交货周期,优质放心的售后服务。我们致力于为您提供有价值的产品和服务,在意您的成功!
产品描述
Matched-Pair Antibody Set for ELISA of human Antithrombin antigen (ATIII). Sufficient reagents to perform 5 x 96 well plates.Antithrombin, also known as Antithrombin III (ATIII), is a member of the SERPIN family of proteinase inhibitors. It is the primary inhibitor of thrombin in plasma. It is produced in the liver and circulates in plasma at ~200ug/ml (~3.5uM). Antithrombin inhibits a broad spectrum of serine proteases including thrombin, activated forms of Factor X, Factor IX, Factor XI, Factor XII, as well as kallikrein, plasmin and urokinase. Enzyme inhibition by antithrombin occurs through proteolytic cleavage at Arg385-Ser386 and subsequent rapid formation of a stable, inactive 1/1 enzyme-antithrombin complex. Heparin has a profound accelerating effect on the inhibitory activity of antithrombin towards some enzymes. For example, the rate of inhibition of thrombin and activated Factor X is increased 1000-fold in the presence of optimal concentrations of heparin. Whereas heparin has relatively little effect on the inhibition rate of activated Factor XI, activated Factor XII and kallikrein. Antithrombin is a single chain molecule with a molecular weight of 59kD. Interaction with thrombin results in an SDS-stable thrombin-antithrombin complex of 96kD.Principle of Sandwich-style ELISA:Affinity-purified antibody to ATIII is coated onto the wells of a microtiter plate. The plates are washed. Plasma or other fluids containing ATIII are applied. The coated antibody will capture the ATIII in the sample. After washing the plate to remove unbound material, a second anti-ATIII antibody labeled with peroxidase is added to the plate to bind to the captured ATIII. After washing the plate to remove unbound labeled antibody, the peroxidase activity is expressed by incubation with o-phenylenediamine (OPD). After a fixed development time, the reaction is quenched with the addition of sulfuric acid. The color produced is quantified using a microplate reader. The color generated is proportional to the concentration of ATIII present in the sample.Kit Components:A2298-19A: Capture Antibody, 1x500ul (Yellow cap). Polyclonal affinity purified anti-ATIII, 50% glycerol solution. Suitable for use as coating antibody. A2298-19B: Detection Antibody (HRP), 5x10ml. Pre-diluted polyclonal anti-ATIII. Labeled with peroxidase. Suitable for use as detection antibody.Quantity:Sufficient reagents for 5x96 well plates.Storage and Stability:May be stored at 4°C. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
产品特点
针对Antithrombin (ATIII) BioAssay ELISA Kit (Antibodies only)该产品的特点优势,欢迎查阅官网提供的产品说明书。
保存建议
建议收到Antithrombin (ATIII) BioAssay ELISA Kit (Antibodies only)产品后将其置于-20°C保存。
其他
Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
注意
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