The recombinant CFP (Cyan Fluorescent Protein) is expressed and purified from transformed E. coli using a method that ensures high purity and maximal CFP fluorescence. The protein is suitable as a control reagent for CFP expression studies or as a labeling reagent. Applications include: Use as standards for SDS-PAGE,Western blot analysis of CFP transfected cells,label other proteins,calibration of fluorometers and flow cytometers,fluorescence microscope,or microinjection of CFP into cells and tissues,etc.
应用类型
Western blot analysis (0.5-4 ug/ml). However, the optimal conditions should be determined individually. Recombinant CFP (Cat.# 4996-100) can be used a positive control.
免疫原
E. coli expressed CFP
来源宿主
兔
反应性
经检测验证,可与所有种属来源的CFP蛋白发生免疫反应.
保存建议
提供的CFP抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA和0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该CFP抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.