Green fluorescent protein (GFP) is a spontaneously fluorescent protein isolated from pacific jellyfish,Aequorea victoria. It transduces the blue chemiluminescence into green fluorescent light. Since the molecular cloning of GFP cDNA and demonstration of GFP as a functional transgene,GFP has become a powerful tool with exciting applications in developmental,cell and molecular biology. GFP fluorescence is not species specific and can be expressed in bacteria,yeast,plant and mammalian cells. GFP can fuse with proteins of interest without interfering significantly with their assembly and function.
应用类型
Western blotting (0.5-4 ug/ml) and in immunoprecipitation (10-20 ug/ml). However, the optimal conditions should be determined individually. The antibody reacts with GFP, and its variants EGFP, RFP, YFP, and CFP, etc. Recombinant EGFP (Cat.# 4999-100), RFP (Cat.# 4997-100), YFP (Cat.# 4998-100) and CFP (Cat.# 4996-100) can be used as positive controls.
免疫原
Recombinant EGFP
来源宿主
兔
反应性
经检测验证,可与所有种属来源的GFP蛋白发生免疫反应.
保存建议
提供的GFP抗体产品为100 ug 无色溶液,其浓度为0.2 mg/ml,溶解于包含有30%甘油, 0.5% BSA, 0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该GFP抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.