A variety of plasmids contain DNA that encodes an N-terminal tag consisting of six histidine (His) residues,followed by an extended multiple cloning sites. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by a single step chromatography. As is the case with other protein tags,this polyhistidine tag can be cleaved at sites recognized by enzymes such as thrombin and enterokinases to isolate the protein of interest.
应用类型
Western blotting (1 ug/ml), immunoprecipitation (40 ug/ml), and immunocytochemistry (2 ug/ml). However, the optimal conditions should be determined individually. The antibody detects His-tagged recombinant proteins.
免疫原
6xHis synthetic peptide
来源宿主
兔
反应性
经检测验证,可与所有种属来源的His-Tag蛋白发生免疫反应.
保存建议
提供的His-Tag抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有50%甘油, 1% BSA, 0.02%硫柳汞的PBS, pH 7.2),缓冲液中.请将该His-Tag抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.