Glutathione S-transferase (GST) is a widely used protein fusion partner,since it provides an easily detectable Tag and also a simple purification process with little effect on the biological function of the protein of interest.
应用类型
Western blotting (0.5-4 ug/ml) and Immunohistochemistry (10-20 ug/ml, frozen & paraffin). Recombinant GST-GSK-3 fusion protein (Cat.#: 7003-100) can be used as a positive control. However, the optimal conditions should be determined individually.
免疫原
GST fusion protein
来源宿主
兔
反应性
经检测验证,可与所有种属来源的GST-Tag蛋白发生免疫反应.
保存建议
提供的GST-Tag抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA, 0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该GST-Tag抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.