Epitope tags are useful for the labeling and detection of proteins using immunoblotting,immunoprecipitation,and immunostaining techniques. The HA tag is derived from an epitope of the influenza hemagglutinin protein,which has been extensively used as a general epitope tag in expression vectors.
应用类型
Western blotting (0.5-4 ug/ml), immunoprecipitation (10-20 ug/ml), and immunocytochemistry (10-20 ug/ml). However, the optimal conditions should be determined individually. The antibody detects HA-tag fused to the C-terminus or N-terminus of targeted proteins in transfected mammalian cells.
免疫原
Synthetic peptide (YPYDVPDYA) derived from the human influenza hemaglutinin protein
来源宿主
小鼠
反应性
经检测验证,可与所有种属来源的HA-Tag蛋白发生免疫反应.
保存建议
提供的HA-Tag抗体产品为200 ug 无色溶液,其浓度为1.0 mg/ml,溶解于包含有50%甘油, 1% BSA, 0.02%硫柳汞的PBS, pH 7.2),缓冲液中.请将该HA-Tag抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.