Epitope tags are usually for the labeling and detection of proteins using immunoblotting,immunoprecipitation and immunostaining techniques. Due to their small size,they are unlikely to affect the tagged protein’s biochemical properties. The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria,yeast,insect and mammalian systems.
应用类型
Western blotting (1-2 ug/ml), immunoprecipitation (20-40 ug/ml), and immunocytochemistry (10-20 ug/ml). However, the optimal conditions should be determined individually. The antibody detects Myc-Tag proteins overexpressed in cells. It does not detect endogenous levels of the transcription factor c-Myc.
免疫原
Synthetic peptide corresponding to residues surrounding amino acid 413 of human c-Myc
来源宿主
兔
反应性
经检测验证,可与所有种属来源的Myc-Tag蛋白发生免疫反应.
保存建议
提供的Myc-Tag抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有50%甘油, 1% BSA, 0.02%硫柳汞的PBS, pH 7.2),缓冲液中.请将该Myc-Tag抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.