Since the molecular cloning of GFP cDNA and demonstration of GFP as a functional transgene,GFP has become a powerful tool with exciting applications in developmental,cell and molecular biology. GFP fluorescence is not species specific and can be expressed in bacteria,yeast,plant and mammalian cells. GFP can also fuse with proteins of interest without interfering significantly with their assembly and function.
应用类型
Western blotting (0.5-4 ug/ml), immunoprecipitation (2-8 ug/ml), immunofluorescence (10-20 ug/ml) and Immunohistochemistry (10-20 ug/ml). However, the optimal conditions should be determined individually. The antibody recognizes wild-type GFP and its derivatives such as EGFP, EBFP, ECFP, and EYFP, etc. Performs well in Western blot analysis (0.5 to 4 ug/ml), immunoprecipitation, and Immunohistochemistry. However, the optimum conditions should be determined individually.
免疫原
Recombinant EGFP expressed from E. col
来源宿主
小鼠
反应性
经检测验证,可与所有种属来源的GFP蛋白发生免疫反应.
保存建议
提供的GFP抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA, 0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该GFP抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.