Since the molecular cloning of GFP cDNA and demonstration of GFP as a functional transgene,GFP has become a powerful tool with exciting applications in developmental,cell and molecular biology. YFP is the recent discovered protein that has the similar function and applications as GFP. Either GFP or YFP fluorescence is not species specific and can be expressed in bacteria,yeast,plant and mammalian cells. GFP or YFP can fuse with proteins of interest without interfering significantly with their assembly and function.
应用类型
Western blotting (0.5-4 ug/ml), immunoprecipitation (10-20 ug/ml) and immunofluorescence. However, the optimal conditions should be determined individually.
免疫原
Recombinant YFP expressed from E. coli
来源宿主
兔
反应性
经检测验证,可与所有种属来源的YFP蛋白发生免疫反应.
保存建议
提供的YFP抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA, 0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该YFP抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.