- 中文名称
genesig Real-Time PCR Detection Kit for Rickettsia (all species), (Citrate synthase gltA) gene), Standard
- 英文名字
- genesig Real-Time PCR Detection Kit for Rickettsia (all species), (Citrate synthase gltA) gene), Standard
- 供应商
- USBiological
- 产品货号
- 349422
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The genesig® Kit for Rickettsia (all species) genomes is designed for the in vitro quantification of Rickettsia genomes. The kit is designed to have the broadest detection profile possible whilst remaining specific to the Rickettsia genome.This kit has been designed for the specific and exclusive in vitro quantification of Rickettsia species within this genus. A highly conserved sequence within the gltA gene has previously been shown to be a good target sequence in other clinical real time PCR based studies (J. Stenos et.al., 2005). The primers and probe sequences in this kit have 100% homology with a broad range of clinically relevant reference sequences based on a comprehensive bioinformatics analysis.genesig Standard Kit:Product features:• Exceptional value for money• Rapid detection of all clinically relevant subtypes• Positive copy no. standard curve for quantification• Highly specific detection profile• High priming efficiency• Broad dynamic detection range (>6 logs)• Sensitive to < 100 copies of target• Accurate controls to confirm findingsSTANDARD KIT COMPONENTS:• Rickettsia specific primer/probe mix (150 reactions BROWN); FAM labelled• Rickettsia positive control template (for Standard curve RED) • RNAse/DNAse free water (WHITE) for resuspension of primer/probe mixes• Template preparation buffer (YELLOW) for resuspension of positive control template and standard curve preparationREAGENTS AND EQUIPMENT TO BE SUPPLIED BY THE USER:• Real-Time PCR Instrument• DNA extraction kitThis kit is recommended for use with genesig EASY DNA/RNA Extraction kit. However, it is designed to work well with all processes that yield high quality DNA with minimal PCR inhibitors.• oasigTM Lyophilised or PrecisionPLUSTM 2 x qPCR MastermixThis kit is designed to work well with all commercially available Mastermixes. However, we recommend the use of oasigTM or PrecisionPLUSTM 2x qPCR MasterMix.• Pipettors and Tips• Vortex and centrifuge• Thin walled 1.5 ml PCR reaction tubesKIT STORAGE AND STABILITY:This kit is stable at room temperature but should be stored at -20°C on arrival. We do not recommend using the kit after the expiry date stated on the pack. Once the lyophilized components have been re-suspended, unnecessary repeated freeze/thawing should be avoided. The kit is stable for six months from the date of resuspension under these circumstances.If a standard curve dilution series is prepared this can be stored frozen for an extended period. If you see any degradation in this serial dilution a fresh standard curve can be prepared from the positive control.SUITABLE SAMPLE MATERIAL:All kinds of sample material suited for PCR amplification can be used. Please ensure the samples are suitable in terms of purity, concentration, and DNA integrity. Always run at least one negative control with the samples. To prepare a negative control, replace the template DNA sample with RNAse/DNAse free water.DYNAMIC RANGE OF TEST:Under optimal PCR conditions genesig® Rickettsia detection kits have very high priming efficiencies of >95% and can detect less than 100 copies of target template.PRODUCT MANUAL:http://www.genesig.com/assets/files/rickettsia_std.pdfINSTRUMENTATION REQUIREMENTS:Designed for use with real-time PCR instruments. . For use in genesig® q16, look for the “Easy” nomenclature. ABOUT THE genesig® KITS:genesig® kits are sold for research use only and are not licensed for diagnostic procedures.Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative Real-Time PCR kits for the detection and simultaneous quantification of numerous significant pathogens. A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented Real-Time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above. Packaged, optimized and ready to use.DNA/RNA EXTRACTION:genesig® Easy DNA/RNA extraction kits are a simple to use, low cost and powerful extraction technology suitable for virtually all sample types.
- 产品特点
- 针对genesig Real-Time PCR Detection Kit for Rickettsia (all species), (Citrate synthase gltA) gene), Standard该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到genesig Real-Time PCR Detection Kit for Rickettsia (all species), (Citrate synthase gltA) gene), Standard产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
-
- 注意
-
该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
-
