Rad17 is an essential component of cell cycle checkpoints activated by DNA damage and disruption of DNA replication. DNA lesions such as single- and double-strand breaks,inter- and intra-strand cross-links,and base modifications hamper transcription and replication,resulting in cell cycle arrest,apoptosis,and mutagenesis. Phosphorylation of Rad17 at Ser635 and Ser645 is a critical early event during checkpoint signaling in DNA damaged cells.
应用类型
Western blotting (0.5-4 ug/ml). However, the optimal concentrations should be determined individually. The antibody recognizes the mature forms of Rad17 (~55 kDa and ~45 kDa) of human, mouse and rat origins. Reactivity to other species has not been tested.
免疫原
Synthetic peptide surrounding amino acid 645 of human Rad17
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠种属来源的Rad17蛋白 发生免疫反应.
保存建议
提供的Rad17抗体 产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA和0.01%硫柳汞的PBS缓冲液中.请将该Rad17抗体 产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.