Rab5 is a 24 kDa GTP-binding protein that is localized to the cytoplasmic side of the plasma membrane,clathrin-coated vesicles,and early endosomes. Rab5 is believed to regulate vesicle fusion through a cycle of GDP/GTP exchange and GTP hydrolysis. The different guanine nucleotide binding states of rab5 is postulated to affect its ability to associate or dissociate with membranes during endocytotic membrane traffic. Its GTP-bound form,which represents the active form of Rab5,associates with membrane and regulates vesicle docking and fusion.
应用类型
Western blotting (0.5-4 ug/ml), immunoprecipitation and immunhistochemistry. However, the optimal concentrations should be determined individually. The antibody recognizes 26 kDa Rab5 of human, mouse, monkey, bovine and rat origins. Reactivity to other species has not been tested.
免疫原
Synthetic peptide surrounding amino acid 185 of human Rab5
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠、猴、牛种属来源的Rab5蛋白发生免疫反应.
保存建议
提供的Rab5抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA和0.01%硫柳汞的PBS缓冲液中.请将该Rab5抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.