SAPK/Erk kinase (SEK1),also known as MKK4 or Jun kinase kinase (JNKK),activates the MAP kinase homologues SAPK and JNK in response to various cellular stresses and inflammatory cytokines. Activation of SEK1 occurs through phosphorylation of serine and threonine residues at positions 257 and 261,respectively,by MEKK. Like MEK,SEK is a dual-specificity protein kinase that phosphorylates SAPK/JNK at a conserved T*PY* site in its activation loop. Phosphorylation by Akt at Ser80 inhibits SEK1 and suppresses the stress-activated signal transduction.
应用类型
Western blotting (1-4 ug/ml). However, the optimal concentrations should be determined individually. The antibody recognizes phosphorylated SEK1/MKK4/JKK1 of human, mouse, and rat origins.
免疫原
Synthetic peptide surrounding Ser80 of human SEK1/MKK4