Membrane receptor signaling by various ligands induces activation of Jak kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN- and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6,it has been shown that Stat3B appears to be activated by both while Stat3 is activated by EGF,but not by IL-6. Highest expression of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 is activated by prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways.
应用类型
Western blotting (0.5-4 ug/ml). However, the optimal concentrations should be determined individually. The antibody recognizes ~97 kDa Stat4 in samples from human, mouse, and rat origins. A ~60 kDa band can also be detected, presumably to be the cleavage fragment of Stat4.
免疫原
Synthetic peptide surrounding amino acid 732 of rat Stat4
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠种属来源的Stat4蛋白发生免疫反应.
保存建议
提供的Stat4抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA和0.01%硫柳汞的PBS缓冲液中.请将该Stat4抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.