Membrane receptor signaling by various ligands induces activation of Jak kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN- and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6,it has been shown that Stat3B appears to be activated by both while Stat3 is activated by EGF,but not by IL-6. Highest expression of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 is activated by prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways.
应用类型
Western blotting (1-4 ug/ml). However, the optimal concentrations should be determined individually. The antibody recognizes ~113 kDa phosphorylated Stat2 (Tyr689) of human and mouse origins. Reactivity to other species has not been tested.
免疫原
Synthetic peptide corresponding to resides around amino acid 686 of mouse Stat2
来源宿主
兔
反应性
经检测验证,可与人、小鼠种属来源的Phospho-Stat2蛋白发生免疫反应.
保存建议
提供的Phospho-Stat2抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有50%甘油, 1% BSA和0.02%硫柳汞的PBS缓冲液中.请将该Phospho-Stat2抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.