Smad proteins,the mammalian homologs of the Drosophila Mothers against dpp (Mad),have been implicated as downstream effectors of TGFB/BMP signaling. Smad1,Smad5,and Smad8 are effectors of BMP2 and BMP4 function while Smad2 and Smad3 are involved in TGF-B and activin-mediated growth modulation. Smad4 has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFB signaling by interfering with TGFB-mediated phosphorylation of other Smad family members.
应用类型
Western blotting (1-4 ug/ml). However, the optimal concentrations should be determined individually. The antibody recognizes ~60 kDa Smad5 of human, mouse, and rat origins. Reactivity to other species has not been tested.
免疫原
Synthetic peptide corresponding to amino acids residues around 252 of human Smad5
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠种属来源的Smad5蛋白发生免疫反应.
保存建议
提供的Smad5抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有50%甘油, 1% BSA和0.02% 叠氮钠的PBS缓冲液中.请将该Smad5抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.