Smad proteins,the mammalian homologs of the Drosophila Mothers against dpp (Mad),have been implicated as downstream effectors of TGFB/BMP signaling. Smad1,Smad5,and Smad8 are effectors of BMP2 and BMP4 function while Smad2 and Smad3 are involved in TGF-B and activin-mediated growth modulation. Smad4 has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFB signaling by interfering with TGFB-mediated phosphorylation of other Smad family members.
应用类型
Western blotting (0.5-4 ug/ml). However, the optimal concentrations should be determined individually. The antibody recognizes ~68 kDa Smad4 from samples of of human, mouse, and rat origins. Reactivity to other species has not been tested.
免疫原
Synthetic peptide corresponding to amino acids residues around 281 of human Smad4
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠种属来源的Smad4蛋白发生免疫反应.
保存建议
提供的Smad4抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA和0.01%硫柳汞的PBS缓冲液中.请将该Smad4抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.