Inherited mutations in the MSH2 and MLH1 homologs of the bacterial DNA mismatch repair genes MutS and MutL were initially demonstrated at high frequency in HNPCC and were shown to be associated with microsatellite instability. The demonstration that 10-45% of pancreatic,gastric,breast,ovarian and small cell lung cancers also display microsatellite instability has been interpreted to suggest that DNA mismatch repair is not restricted to HNPCC tumors but is a common feature in tumor initiation or progression. Two additional homologs of the prokaryotic MutL gene,designated PMS1 and PMS2,have been identified and shown to be mutated in the germline of HNPCC patients.
应用类型
Western blotting (0.5-4 ug/ml), immunoprecipitation (10-20 ug/ml) and Immunohistochemistry (10-20 ug/ml). However, the optimal concentrations should be determined individually.
免疫原
Synthetic peptide surrounding amino acid 846 of human PMS2
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠种属来源的PMS2蛋白 发生免疫反应.
保存建议
提供的PMS2抗体 产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA和0.01%硫柳汞的PBS缓冲液中.请将该PMS2抗体 产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.