PKGs are cyclic GMP-dependent protein kinases (also designed cGKs) that are classified into two types,PKGI and PKGII. Studies have shown that cGKs and cyclic AMP-dependent kinases (cAKs) are highly homologous protein kinase families with similar substrate specificities. Phosphorylation of cellular proteins by both families of kinases leads to alterations in calcium mobilization,protein phosphatase activity,ion channel function,gene transcription,smooth muscle contractility and platelet aggregation. However,recent studies using mice deficient in PKGs have shown that cGMP kinases regulate very specifically distinct pathways which are separate from those used by cyclic AMP kinases.
应用类型
The antibody can be used for Western blotting (0.5-4 ug/ml). However, the optimal conditions should be determined individually. The antibody recognizes 75 and 85 kDa PKG, corresponding to the molecular mass of two PKG isoforms.
免疫原
Synthetic peptide near the N-terminus of human PKG
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠、鸡、仓鼠、牛、兔种属来源的PKG蛋白 发生免疫反应.
保存建议
提供的PKG抗体 产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA和0.01%硫柳汞的PBS缓冲液中.请将该PKG抗体 产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.