PKC (Protein kinase C) is a calcium-dependent and phospholipid-dependent enzyme that is activated in vivo by the lipid diacylglycerol. PKC has been shown to be involved in signal transduction and various aspects of neoplastic transformation. PKC contains an amino terminal regulatory domain and a carboxy terminal catalytic domain joined by a hinge region. Cleavage of protein kinase C in vitro results in the generation of two functional fragments corresponding to these two domains.
应用类型
Western blotting (0.5-4 ug/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 60-75 kDa PKC Bisoforms from samples of human, mouse, rat, pig and bovine origins. A 40 kDa band can also be detected, presumably to be a shorter form of PKC isoforms. Reactivity to other species has not been tested.
免疫原
Synthetic peptide corresponding to the C-terminus of mouse PKC B1
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠种属来源的PKC蛋白发生免疫反应.
保存建议
提供的PKC抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA和0.01%硫柳汞的PBS缓冲液中.请将该PKC抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.