The PAK (p21-activated kinase) family of serine/threonine kinases plays an important role in multiple cellular processes,including cytoskeletal reorganization,MAPK signaling,apoptotic signaling,etc. Several mechanisms that induce PAK activation have been reported. Binding of Rac/cdc42 to the CRIB (or PBD) domain at the N-terminal region of PAK causes autophosphorylation and conformational change of PAK. More recently identified members PAK4,PAK5 and PAK6 have lower sequence similarity with PAK1-3 in the regulatory N-terminal region. It has been demonstrated that phosphorylation of serine 474 of PAK4 may play a pivotal role in the activity and function of PAK4 kinase
应用类型
Western blot analysis (0.5-4 ug/ml). However, the optimal conditions should be determined individually. The antibody recognizes ~60-70 kDa Pak4 in samples of human, mouse, and rat origins. The antibody does not cross-react with other Paks. Reactivity to other species has not been tested.
免疫原
Synthetic peptide corresponding to the N-terminus of human Pak4
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠、仓鼠种属来源的PAK4蛋白发生免疫反应.
保存建议
提供的PAK4抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA, 0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该PAK4抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.