The PAK (p21-activated kinase) family of serine/threonine kinases plays an important role in multiple cellular processes,including cytoskeletal reorganization,MAPK signaling,apoptotic signaling,etc. Binding of Rac/cdc42 to the CRIB (or PBD) domain at the N-terminal region of PAK causes autophosphorylation and conformational change of PAK. Phosphorylation of Ser21 of PAK1 or Ser20 of PAK2 regulates its binding with the adaptor protein Nck.
应用类型
Western blotting (0.5-4 ug/ml). However, the optimal concentrations should be determined individually. Jurkat cell lysate (Cat.# 2601-100) can be used as a positive control. The antibody recognizes 60 kDa Pak2 of human, mouse, rat, and rabbit origins. The antibody does not cross-react with Pak1, Pak3, and Pak4-6. Reactivity to other species has not been tested.
免疫原
Synthetic peptide corresponding to residues surrounding amino acid 5 of human Pak2
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠、兔种属来源的PAK2蛋白发生免疫反应.
保存建议
提供的PAK2抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA和0.01%硫柳汞的PBS缓冲液中.请将该PAK2抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.