Both p44 and p42 MAP kinases (Erk1 and Erk2) function in a protein kinase cascade that plays a critical role in the regulation of cell growth and differentiation. Activation of MAP kinases occurs through phosphorylation of threonine and tyrosine (202 and 204 of human MAP kinase [Erk1] or 183 and 185 of rat Erk2) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK). Both kinases are known to weakly autophosphorylate on tyrosine.
应用类型
Western blotting (0.5-4ug/ml), immunoprecipitation(10-20 ug/ml),and Immunohistochemistry (10-20 ug/ml). However, the optimal concentrations should be determined individually. The antibody recognizes Phosphorylated p42 and p44 (Erk1 and Erk2).
免疫原
Synthetic phospho-peptide surrounding Thr202/Tyr204 of human P44MAP kinase
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠种属来源的Phospho-Erk1/2蛋白发生免疫反应.
保存建议
提供的Phospho-Erk1/2抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有50%甘油, 1% BSA和0.02%硫柳汞的PBS缓冲液中.请将该Phospho-Erk1/2抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.