uPAR (Urokinase plasminogen activator receptor) is a 55 kDa glycoprotein I-anchored surface receptor specific for urokinase plasminogen activator (uPA). Upon binding to uPAR,uPA converts the surface bound,large serum B-globulin,plasminogen to plasmin. Plasmin,which is also designated fibrinolysin,is a trypsin-like enzyme that acts on Arg-Lys bonds and induces pericellular proteolysis in fibrin and fibrinogen,and thereby contributes to the systematic activation of the coagulation cascade. uPA and uPAR are known to be overexpressed in mesenchymal and epithelial tumor cells and are required for tumor invasion and metastasis.
应用类型
Western blotting (0.5-2 ug/ml), immunoprecipitation (5-10 ug/ml), and neutralization (2-4 ug/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 55 kDa human uPAR. Reactivity to other species has not been tested.
免疫原
E. coli expressed recombinant human uPAR
来源宿主
山羊
反应性
经检测验证,可与人种属来源的uPAR蛋白发生免疫反应.
保存建议
提供的uPAR抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA和0.01%硫柳汞.的PBS缓冲液中.请将该uPAR抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.