MAP kinase-activated protein kinase-2 (MAPKAPK-2),also known as p45 hsp27 kinase,is a 45-54 kDa serine-threonine protein kinase. MAPKAPK-2 is rapidly phosphorylated and activated in response to stress,cytokines,and chemotactic factors. One of the major substrates of MAPKAPK-2 is hsp27,which stimulates actin polymerization in order to facilitate recovery from destruction of cytoskeleton during cellular stresses. Two isoforms are produced due to alternative splicing of the same gene which differs in their C-terminals. This antibody recognizes both isoforms.
应用类型
Western blotting (1 ug/ml). However, the optimal conditions should be determined individually. The antibody detects phosphorylated MAPKAPK-2 (Thr334) of human and mouse origins. Reactivity to other species has not been tested.
免疫原
Synthetic peptide corresponding to residues surrounding Thr334 of human MAPKAPK-2
来源宿主
兔
反应性
经检测验证,可与人、小鼠种属来源的Phospho-MAPKAPK-2蛋白发生免疫反应.
保存建议
提供的Phospho-MAPKAPK-2抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有50%甘油, 1% BSA, 0.02%硫柳汞的PBS (pH 7.2)缓冲液中.请将该Phospho-MAPKAPK-2抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.