LIM kinases (LIMK1 and LIMK2) are serine/threnine kinases that have two zinc finger motifs,known as LIM motifs in their N-terminal regulatory domain. LIM kinases are involved in actin cytoskeleton regulation through Rho-family GTPases and downstream kinases PAKs and ROCK. PAK1 and ROCK phosphorylate LIMK1 and LIMK2,which increases the activity of the kinases. Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the N-terminus of Cofilin.
应用类型
Western blotting (1-4 ug/ml). However, the optimal conditions should be determined individually. The antibody detects 70 kDa LIMK1 protein. It does not cross-react with LIMK2.
免疫原
Synthetic peptide corresponding to the C-terminus of human LIMK1
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠种属来源的LIMK1蛋白发生免疫反应.
保存建议
提供的LIMK1抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有50%甘油, 1% BSA, 0.02%硫柳汞的PBS (pH 7.2)缓冲液中.请将该LIMK1抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.