MLK3 （Mixed lineage kinase 3） is a serine/threonine kinase that has an amino-terminal SH3 domain followed by the kinase domain and two leucine zippers，a cdc42/Rac1 binding （CRIB） domain and several other domains/motifs at the carboxy-terminal region. MLK3 functions as a MAPKKK of the SAPK/JNK stress pathway by directly phosphorylating SEK1/MKK4 and MKK7，although it is controversial whether MLK3 is involved in p38 stress pathway activation. MLK3 also functions as an IkB kinase and mediates the activation of the transcriptional factor NF-kB stimulated by CD3/CD28，suggesting a role for MLK3 in immune and inflammatory responses.
应用类型
Western blotting （0.5-4 ug/ml）. However， the optimal concentrations should be determined individually. The antibody recognizes 92 kDa MLK3 of human， mouse， and rat origins. It does not cross-react with MLK1， MLK2 and other mixed lineage kinases.
免疫原
Synthetic peptide mapping to the C-terminus of human MLK3.
来源宿主
兔
反应性
经检测验证，可与人、小鼠、大鼠种属来源的MLK3蛋白发生免疫反应.
保存建议
提供的MLK3抗体产品为100 ug 无色溶液，其浓度为0.5 mg/ml，溶解于包含有50%甘油， 1% BSA和0.02%硫柳汞的PBS缓冲液中.请将该MLK3抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
