eNOS (Endothelial nitric-oxide synthase) is an important enzyme in the cardiovascular system. eNOS is regulated by phosphorylation at multiple sites. The two most thoroughly studied sites are the activation site Ser1177 and the inhibitory site Thr495. Several protein kinases including Akt/PKB,PKA and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli. In contrast,bradykinin and hydrogen peroxide activate eNOS activity by promoting Thr495 dephosphorylation.
应用类型
Western blotting (0.5-4 ug/ml). However, the optimal conditions should be determined individually. The antibody recognizes 140 kDa eNOS of human, mouse, and rat origins. Reactivity to other species has not been tested.
免疫原
Synthetic peptide surrounding amino acid 602 of rat eNOS
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠种属来源的eNOS蛋白 发生免疫反应.
保存建议
提供的eNOS抗体 产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA和0.01%硫柳汞的PBS缓冲液中.请将该eNOS抗体 产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.