NPM (Nucleophosmin) is an abundant phosphoprotein primarily found in nucleoli. It has been implicated in several distinct cellular functions,including assembly and transport of ribosomes,cytoplasmic/nuclear trafficking,regulation of DNA polymerase alpha activity,centrosome duplication and molecular chaperoning activities. The NPM gene is also known for its fusion with the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. The NPM portion contributes to transformation by providing a dimerization domain,which results in activation of the fused kinase.
应用类型
Western blotting (1-2 ug/ml) and Immunohistochemistry (10-20 ug/ml). However, the optimal concentrations should be determined individually.
免疫原
Synthetic peptide corresponding to amino acids surrounding the N-terminus of human NPM
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠种属来源的NPM蛋白发生免疫反应.
保存建议
提供的NPM抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有50%甘油, 1% BSA和0.02%硫柳汞的PBS缓冲液中.请将该NPM抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.