IRS-1 (Insulin receptor substrate-1) is phosphorylated in response to stimulation of cells by insulin. Upon ligand-induced phosphorylation,IRS-1 immediately associates with a series of SH2 domain-containing signaling intermediates such as PI3-kinase,GRB2,Syp and Nck. The level of diversity in structure and function of the SH2-containing proteins which binds activated IRS-1 is important for responsiveness to insulin. IRS phosphorylation elicits the growth and metablic effects of insulin growth factors and insulin by allowing glucose uptake,glycogen synthesis and mitogenic activity. IRS-1 migrates between 170 and 185 kDa and is expressed in most cell types.
应用类型
Western blotting (0.5-4 ug/ml). However, the optimal conditions should be determined individually
免疫原
Synthetic peptide mapping to the C-terminus of human IRS-1
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠种属来源的IRS-1蛋白发生免疫反应.
保存建议
提供的IRS-1抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA和0.01%硫柳汞的PBS缓冲液中.请将该IRS-1抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.