ILKs (Integrin-linked kinases) in combination with integrins and growth factors regulate cell survival,cell cycle,cell-cell adhesion and cell motility. ILK functions as a scaffold bridging the extra-cellular matrix (ECM) and growth factor receptors to the actin cytoskeleton through interactions with integrin,PINCH (which links ILK to the RTKs via Nck2),CH-ILKBP and affixin. ILK phosphorylates several cellular targets including Akt,GSK-3,myosin light chain 2,as well as affixin. These phosphorylation events are key regulatory steps in modulating the activities of the targets.
应用类型
Western blotting (0.5-4 ug/ml), Immunohistochemistry (10-20 ug/ml, frozen & paraffin). However, the optimal concentrations should be determined individually. The antibody recognizes 59 kDa of ILK1 from samples of human, mouse, and rat origins. Reactivity to other species has not been determined.
免疫原
Synthetic peptide corresponding to the C-terminus of human ILK-1
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠种属来源的ILK1蛋白 发生免疫反应.
保存建议
提供的ILK1抗体 产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA和0.01%硫柳汞的PBS缓冲液中.请将该ILK1抗体 产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.