Chemokines play important roles in inflammation and critical for the recruitment of effector immune cells to sites of infection. Chemokines activate leukocytes by binding to G protein coupled receptors. The ever-growing chemokine receptor subtypes can be divided into 2 major groups,CXCR and CCR,based on the 2 major classes of chemokines. One of the CCR receptors,CCR1,is expressed on neutrophils,monocytes,lymphocytes,and eosinophils and binds the leukocyte chemoattractant and hemopoiesis regulator macrophage-inflammatory protein (MIP-1 ),eotaxin,as well as several other related chemokines. Mice lacking the chemokine receptor CCR1 have defects in neutrophil trafficking and proliferation.
应用类型
Western blotting (0.5-4 ug/ml). However, the optimal conditions should be determined individually. The antibody detects ~42 kDa of CCR2 in samples from human, mouse and rat origins. Reactivity to other species has not been determined.
免疫原
Synthetic peptide surrounding to amino acid 353 of rat CCR2
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠种属来源的CCR2蛋白 发生免疫反应.
保存建议
提供的CCR2抗体 产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油和0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该CCR2抗体 产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.