Chemokines play an important role in inflammation and are critical for the recruitment of effector immune cells to sites of infection. Chemokines activate leukocytes by binding to G protein coupled receptors. The ever-growing chemokine receptor subtypes can be divided into 2 major groups,CXCR and CCR,based on the 2 major classes of chemokines. One of the CCR receptors,CCR1,is expressed on neutrophils,monocytes,lymphocytes,and eosinophils and binds the leukocyte chemoattractant and hemopoiesis regulator macrophage-inflammatory protein (MIP-1 ),eotaxin,as well as several other related chemokines. Mice lacking the chemokine receptor CCR1 have defects in neutrophil trafficking and proliferation.
应用类型
Western blotting (0.5-4 ug/ml). However, the optimal concentrations should be determined individually. The anti-CCR1 antibody recognizes 42 kDa human and monkey CCR1 and in a lesser extent the mouse CCR1 in Western blot analysis.
免疫原
Synthetic peptide surrounding amino acid 341 of human CCR1
来源宿主
兔
反应性
经检测验证,可与人、小鼠、猴种属来源的CCR1蛋白发生免疫反应.
保存建议
提供的CCR1抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA和0.01%硫柳汞的PBS缓冲液中.请将该CCR1抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.