Caspase-9 is one of the most important caspases among the caspase family members. Upon induction of apoptosis,cytochrome c released from mitochondria associates with procaspase-9/Apaf-1. The complex processes procaspase-9 into a large subunit (35 kDa or 17 kDa) and small (10 kDa) by self-cleavage at D315. Activated caspase-9 further cleaves other caspase members including caspase-3,one of the proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. In addition to self-cleavage,procaspase-9 can also be cleaved in vivo by caspase-3 at D330. The process served as a positive feedback to amplify the apoptotic signal in caspase activation pathway.
应用类型
Western blotting (0.5-4 ug/ml). However, the optimal conditions should be determined individually. Mouse small intestine and rat kidney tissue lysates can be used as positive controls. The antibody detects 45 kDa procaspase-9, 35/37 kDa cleaved large fragment and the 25 kDa fully cleaved caspase-9.
免疫原
Synthetic peptide surrounding amino acid 345 of mouse caspase-9
来源宿主
兔
反应性
经检测验证,可与小鼠、大鼠种属来源的Caspase-9蛋白发生免疫反应.
保存建议
提供的Caspase-9抗体产品为100 ug 无色溶液,其浓度为0.2 mg/ml,溶解于包含有30%甘油, 0.5% BSA, 0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该Caspase-9抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.