Focal adhesion kinase was identified as a 125 kDa substrate for the intrinsic protein tyrosine kinase activity of Src encoded pp60. The deduced amino acid sequence of FAK p125 has shown it to be a cytoplasmic protein tyrosine kinase whose sequence and structural organization are unique as compared to other proteins described to date. Localization of p125 by immunofluorescence suggests that it is primarily found in cellular focal adhesions leading to its designation as focal adhesion kinase (FAK). FAK is concentrated at the basal edge of only those basal keratinocytes that are actively migrating and rapidly proliferating in repairing burn wounds and is activated and localized to the focal adhesions of spreading keratinocytes in culture. Thus,it has been postulated that FAK may have an important in vivo role in the reepithelialization of human wounds. FAK protein tyrosine kinase activity has also been shown to increase in cells stimulated to grow by use of mitogenic neuropeptides or neurotransmitters acting through G protein coupled receptors.
应用类型
Western blotting (1-2 ug/ml), immunoprecipitation (5-20 ug/ml), and immunocytochemistry (10-20 ug/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 125 kDa FAK of human, mouse, and rat origins.
免疫原
Recombinant human FAK
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠种属来源的FAK蛋白发生免疫反应.
保存建议
提供的FAK抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有50%甘油, 1% BSA和0.02%硫柳汞的PBS缓冲液中.请将该FAK抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.