Etk is a member of the Bruton’s tyrosine kinase family. Etk is expressed in a variety of hematopoietic,epithelial and endothelial cells. It participates in multiple signal transduction pathways. Phosphorylation of tyrosine 566 by Src kinase is required for activation of Etk in vivo. In endothelial and epithelial cells,Etk is regulated by FAK through phosphorylation at tyrosine 40.
应用类型
Western blotting (0.5-4 ug/ml). However, the optimal concentrations should be determined individually. Jurkat cell lysate can be used as a positive control. The antibody recognizes 76 kDa Etk from samples of human, mouse, and rat origins.
免疫原
Synthetic peptide surrounding amino acid 43 of human Etk
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠种属来源的Etk/BMX蛋白发生免疫反应.
保存建议
提供的Etk/BMX抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有50%甘油, 1% BSA和0.02%硫柳汞的PBS缓冲液中.请将该Etk/BMX抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.