Heme oxygenase (HO) is a microsomal enzyme that oxidatively cleaves heme,a pro-oxidant,into carbon monoxide and biliverdin. Two heme oxygenase isozymes,termed HO-1 and HO-2,have been identified so far. At the amino acid level,HO-1 and HO-2 are 42% homologous. However,each isozyme is evolutionary highly conserved. HO-1 is the inducible form of the enzyme and is therefore also referred to as stress protein Hsp32. Expression of HO-1 can be induced by a variety of stimuli such as heme,metals,hormones,UV radiation and sulfhydryl depleting agents. In contrast,HO-2 is the constituitive form of the enzyme. It is not inducible by exogeneous stimuli and HO-2 is the most prevalent form in most tissues,except the spleen where HO-1 levels are predominant under normal conditions.
应用类型
Western blotting (0.5-4 ug/ml), in immunoprecipitation (20 ug/ml) and Immunohistochemistry (20 ug/ml, frozen section tested). However, the optimal concentrations should be determined individually. The antibody recognizes ~36 kDa Heme-Oxygenase-2 of human, mouse, rat, hamster, and monkey origins. Blocking peptide (Cat.# 3392BP-50) is available separately.
免疫原
Synthetic peptide surrounding amino acid 34 of human HO-2