Elk-1 is a transcription factor that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors. Elk-1 is phosphorylated by MAP kinase pathways and appears to be a direct target of activated MAP kinase. Biochemical studies indicate that Elk-1 is a good substrate for MAP kinase. The kinetics of Elk-1 phosphorylation and activation correlate with MAP kinase activity. Other studies have shown that Elk-1 (Ser383) is also a target of the stress-activated kinase SAPK/JNK.
应用类型
Western blotting (0.5-4 ug/ml). However, the optimal concentrations should be determined individually. The antibody detects 62 kDa phosphorylated Elk-1 (Ser383) of human, mouse and rat origins.
免疫原
Synthetic peptide surrounding amino acid 383 of human Elk-1
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠种属来源的Phospho-Elk-1蛋白发生免疫反应.
保存建议
提供的Phospho-Elk-1抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有50%甘油, 1% BSA和0.02%硫柳汞的PBS缓冲液中.请将该Phospho-Elk-1抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.