Elk-1 is a transcription factor that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors. Elk-1 is phosphorylated by MAP kinase pathways and appears to be a direct target of activated MAP kinase. Biochemical studies indicate that Elk-1 is a good substrate for MAP kinase. The kinetics of Elk-1 phosphorylation and activation correlate with MAP kinase activity. Other studies have shown that Elk-1 (Ser383) is also a target of the stress-activated kinase SAPK/JNK.
应用类型
Western blotting (0.5-4 ug/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 62 kDa Elk-1 of human, mouse and rat origins.
免疫原
Synthetic peptide surrounding amino acid 383 of human Elk-1