CaMKII is a member of calcium/calmodulin-activated kinase,functioning in neural synaptic stimulation and T-cell receptor signaling. CaMKII has catalytic and regulatory domains. The binding of Ca++/calmodulin to its regulatory domain releases its autoinhibitory effect and activates the kinase. The activated CaMKII further autophosphorylates itself at Thr286 to render the kinase constitutively active.
应用类型
Western blotting at 1-2 ug/ml. However, the optimal concentrations should be determined individually. The anti-Phospho-CaMKII antibody recognizes only the phosphorylated CaMKII (Thr286) in samples from human, mouse, and rat origins.
免疫原
Synthetic phospho-peptide corresponding to residues surrounding Thr286 of human CaMKII
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠种属来源的Phospho-CaMKII蛋白发生免疫反应.
保存建议
提供的Phospho-CaMKII抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有50%甘油, 1% BSA和0.02%硫柳汞的PBS缓冲液中.请将该Phospho-CaMKII抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.