Catenin is one of the key downstream effectors in the Wnt signaling pathway. It has been implicated to play an important role in early embryonic development and tumorigenesis. B-catenin can be destabilized by GSK-3B or other kinases by phosphorylating it at serines 33,37,45 and threonine 41. Mutations of these phosphorylation sites in B-catenin have been found in many tumor cell lines.
应用类型
Western blotting (0.5-4 ug/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 90 kDa phosphorylated B-Catenin (Ser45) of human, mouse, and rat origin.
免疫原
Synthetic phospho-peptide corresponding to residues surrounding Ser45 of human B-Catenin
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠种属来源的Phospho-b-Catenin蛋白发生免疫反应.
保存建议
提供的Phospho-b-Catenin抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有50%甘油, 1% BSA和0.02%硫柳汞的PBS缓冲液中.请将该Phospho-b-Catenin抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.