Cadherins comprise a family of Ca-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. Cadherins consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The most distal of cadherins is though to be responsible for binding specificity,transmembrane domains and carboxy terminal domains. The relative short intracellular domains interact with a variety of cytoplasmic proteins,such as B-catenin,to regulate cadherin function.
应用类型
Western blotting (1-2 ug/ml), immunoprecipitation (10-20 ug/ml), and Immunohistochemistry (10-20 ug/ml). However, the optimal conditions should be determined individually. The antibody detects E- and P-cadherin (~95 kDa). Jurkat cell lysate (Cat.# 2401-100) can be used as a positive control.
免疫原
Synthetic peptide surrounding amino acid 388 of human Cadherin
来源宿主
兔
反应性
经检测验证,可与人、小鼠种属来源的Cadherin蛋白发生免疫反应.
保存建议
提供的Cadherin抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA, 0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该Cadherin抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.