BRCA1 is a breast cancer susceptibility gene that was localized to chromosome 17q. Mutations within this gene are believed to account for approximately 45% of families with high incidence of breast cancer and at least 80% of families with increased incidence of both early-onset breast cancer and ovarian cancer. The BRCA1 gene is expressed in numerous tissues,including breast and ovary,and encodes a predicted protein of 1863 amino acids. Like many other genes involved in familial cancer,BRCA1 appears to encode a tumor suppressor,a protein that acts as a negative regulator of tumor growth.
应用类型
Western blotting (1-4 ug/ml), immunoprecipitation (10-20 ug/ml), and Immunohistochemistry (10-20 ug/ml). However, the optimal conditions should be determined individually.
免疫原
Synthetic peptide surrounding amino acid 1852 of human BRCA1