COX proteins are membrane-associated heme proteins that have cyclooxygenase and peroxidase activities. These enzymes are targets of NSAID (Nonsteroidal anti-inflammatory drugs) such as aspirin. Prostaglandins (PGs) formed by the enzymatic activity of COX-1 are primarily involved in the regulation of homeostatic functions throughout the body,whereas PGs formed by COX-2 primarily mediate pain,fever,and inflammation. COX-1 is constitutively expressed,with particularly high expression in gastrointestinal tissues. COX-2 is induced by cytokines and mitogens and is likely to play a role in inflammatory diseases such as rheumatoid arthritis.
应用类型
Western blotting (1-4 ug/ml), immunoprecipitation, and Immunohistochemistry. However, the optimal concentrations should be determined individually. The antibody recognizes 70 kDa COX-1 of human, mouse and rat origins. It does not cross-react with COX-2.
免疫原
Synthetic peptide corresponding to residues surrounding amino acids 586 of mouse Cox-1
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠种属来源的Cox-1蛋白发生免疫反应.
保存建议
提供的Cox-1抗体产品为100 ug 无色溶液,其浓度为0.2 mg/ml,溶解于包含有50%甘油, 1% BSA和0.02% 叠氮钠的PBS缓冲液中.请将该Cox-1抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.