Transcription factor CREB binds the cAMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters,hormones,membrane depolarization,and growth or neurotrophic factors. Protein kinase A and the calmodulin-dependent protein kinase CaMKII stimulate CREB phosphorylation at Ser133,a key regulatory site controlling transcriptional activity. Phosphorylation at Ser133 is also mediated by p44/42 MAP kinase,p90RSK,p38 MAP kinase and MSK1. CREB appears to play an important role in learning and memory in both flies and mice.
应用类型
Western blotting (0.5-4 ug/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 43 kDa CREB from samples of human, mouse and rat origins.
免疫原
Synthetic peptide surrounding amino acid 109 of rat CREB
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠种属来源的CREB Polyclonal蛋白发生免疫反应.
保存建议
提供的CREB Polyclonal抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA和0.01%硫柳汞的PBS缓冲液中.请将该CREB Polyclonal抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.