The transcription factor ATF-2 (also called CRE-BP1) is a member of the ATF/CREB family of leucine zipper proteins. ATF-2 forms homodimers and heterodimers with c-Jun to initiate CRE-dependent transcription. Phosphorylation of ATF-2 at Thr69 and Thr71 by stress-activated kinases is necessary for transcriptional activation. Myc also induces phosphorylation of ATF-2 at Thr69 and Thr71 to prolong the half-life of ATF-2. ATF-2 also functions as a histone acetyltransferase (HAT) by specifically acetylating histones H2B and H4 in vitro.
应用类型
Western blotting (1-4 ug/ml), immunoprecipitation (10 ug/ml), and Immunohistochemistry (20 ug/ml). However, the optimal conditions should be determined individually. The antibody detects phosphorylated ATF-2. It recognizes both Thr69/Thr71 dually phosphoralated ATF-2 as well as ATF-2 singly phosphorylated ATF-2 in samples of human, mouse, and rat origins.
免疫原
Synthetic phospho-peptide corresponding to residues surrounding Thr71 of human ATF2
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠种属来源的Phospho-ATF-2蛋白发生免疫反应.
保存建议
提供的Phospho-ATF-2抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有50%甘油, 1% BSA, 0.02%硫柳汞的PBS (pH 7.2)缓冲液中.请将该Phospho-ATF-2抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.